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plx301 construct  (Addgene inc)


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    Structured Review

    Addgene inc plx301 construct
    Plx301 Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plx301/pLX301+(Plasmid+%2325895)/bio_rxiv__64898__2026__03__16__712096-216-24-29
    Average 93 stars, based on 66 article reviews
    plx301 construct - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:


    Article Title: RalB uncoupling from exocyst is required for endothelial Weibel-Palade body exocytosis
    Article Snippet: Moua Yanga,b, Alexandra Boye-Doec, Mohammed Alic, Salma A.S. Abosabiec,d,e, Alexandra M. Barrc, Lourdes M. Mendezc, and Anish V. Sharda ,c,* aBloodworks Northwest Research Institute, Seattle, WA 98102; bDivision of Hematology and Oncology, Department of Medicine, University of Washington School of Medicine, Seattle, WA 98102; cSection of Hematology, Yale Cancer Center, Yale University School of Medicine, New Haven, CT 06511; dInstitute of Experimental Biomedicine, University Hospital Würzburg, 97080 Würzburg, Germany; eRudolf Virchow Center for Integrative and Translational Bioimaging, Julius-Maximilians-Universität Würzburg, 97080 Würzburg, Germany

    Article Title: Failure to repair damaged NAD(P)H blocks de novo serine synthesis in human cells
    Article Snippet: .. Both the isoforms were cloned from pcDNA3.1 that contained the human full-length NAXD cDNA (RefSeq ID: NM_001242882.1; GenScript clone ID OHu08429) [ ]. pLX301 was a gift from David Root (Addgene plasmid # 25,895) [ ]. .. The lentiviral particles were generated in human embryonic kidney (HEK) 293 T cells via co-transfection of three plasmids (recombinant pLX301, containing the eGFP, CytoNAXD or MitoNAXD inserts, pCMV-δ8.2, and pCMV-VSVg) using LipofectamineTM 2000 transfection reagent (Invitrogen), as detailed previously [ , ].

    Article Title: <scp>DeSUMOylating</scp> isopeptidase 1 participates in the faithful chromosome segregation and vincristine sensitivity
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 4 of 21 | IKEDA et al. n2t. net/ addge ne: 17357 ; RRID:Addgene_17357)31], was ligated into the SalI–XbaI sites of the pENTR4- no- ccDB. .. The genes in the entry vectors were subcloned into the gateway destination vectors, pLIX402 [a gift from David Root (Addgene plasmid #41394; http:// n2t. net/ addge ne: 41394 ; RRID:Addgene_41394)], pLX301 [a gift from David Root (Addgene plasmid #25895; http:// n2t. net/ addge ne: 25895 ; RRID:Addgene_25895)32], and pLX303 [a gift from David Root (Addgene plasmid #25897; http:// n2t. net/ addge ne: 25897 ; RRID:Addgene_25897)32] lentiviral plasmids using the Gateway LR reaction as per the manufacturer's instructions (Thermo Fisher Scientific). ..

    Article Title: DeSUMOylating isopeptidase 1 participates in the faithful chromosome segregation and vincristine sensitivity
    Article Snippet: To construct pENTR4‐no‐ccDB‐FLAG‐SENP1, a SENP1 fragment harboring SalI–XbaI sites, amplified with primers (5′‐AAAGTCGACACCATGGACTACAAAGACGATGACGACAAGC‐3′ and 5′‐AAATCTAGATCACAAGAGTTTTCGGTGGAGGATCTCCC‐3′) using the Flag‐SENP1 [a gift from Edward Yeh (Addgene plasmid #17357; http://n2t.net/addgene:17357 ; RRID:Addgene_17357) ], was ligated into the SalI–XbaI sites of the pENTR4‐no‐ccDB. .. The genes in the entry vectors were subcloned into the gateway destination vectors, pLIX402 [a gift from David Root (Addgene plasmid #41394; http://n2t.net/addgene:41394 ; RRID:Addgene_41394)], pLX301 [a gift from David Root (Addgene plasmid #25895; http://n2t.net/addgene:25895 ; RRID:Addgene_25895) ], and pLX303 [a gift from David Root (Addgene plasmid #25897; http://n2t.net/addgene:25897 ; RRID:Addgene_25897) ] lentiviral plasmids using the Gateway LR reaction as per the manufacturer's instructions (Thermo Fisher Scientific). ..

    other:

    Article Title: Highly networked immunogen composition
    Article Snippet: Non-limiting examples of lentiviral vectors include pLVX-EF1alpha-AcGFP1-C1 (Clontech Catalog #631984), pLVX-EF1alpha-IRES-mCherry (Clontech Catalog #631987), pLVX-Puro (Clontech Catalog #632159), pLVX-IRES-Puro (Clontech Catalog #632186), pLenti6/V5-DESTTM (Thermo Fisher), pLenti6.2/V5-DESTTM (Thermo Fisher), pLKO.1 (Plasmid #10878 at Addgene), pLKO.3G (Plasmid #14748 at Addgene), pSico (Plasmid #11578 at Addgene), pLJM1-EGFP (Plasmid #19319 at Addgene), FUGW (Plasmid #14883 at Addgene), pLVTHM (Plasmid #12247 at Addgene), pLVUT-tTR-KRAB (Plasmid #11651 at Addgene), pLL3.7 (Plasmid #11795 at Addgene), pLB (Plasmid #11619 at Addgene), pWPXL (Plasmid #12257 at Addgene), pWPI (Plasmid #12254 at Addgene), EF.CMV.RFP (Plasmid #17619 at Addgene), pLenti CMV Puro DEST (Plasmid #17452 at Addgene), pLenti-puro (Plasmid #39481 at Addgene), pULTRA (Plasmid #24129 at Addgene), pLX301 (Plasmid #25895 at Addgene), pHIV-EGFP (Plasmid #21373 at Addgene), pLV-mCherry (Plasmid #36084 at Addgene), pLionII (Plasmid #1730 at Addgene), pInducer10-mir-RUP-PheS (Plasmid #44011 at Addgene).

    Article Title: Methods and compositions for increasing protein expression and/or treating a haploinsufficiency disorder
    Article Snippet: Non-limiting examples of lentiviral vectors include pLVX-EF1alpha-AcGFP1-C1 (Clontech Catalog #631984), pLVX-EF1alpha-IRES-mCherry (Clontech Catalog #631987), pLVX-Puro (Clontech Catalog #632159), pLVX-IRES-Puro (Clontech Catalog #632186), pLenti6/V5-DESTTM (Thermo Fisher), pLenti6.2/V5-DESTTM (Thermo Fisher), pLKO.1 (Plasmid #10878 at Addgene), pLKO.3G (Plasmid #14748 at Addgene), pSico (Plasmid #11578 at Addgene), pLJM1-EGFP (Plasmid #19319 at Addgene), FUGW (Plasmid #14883 at Addgene), pLVTHM (Plasmid #12247 at Addgene), pLVUT-tTR-KRAB (Plasmid #11651 at Addgene), pLL3.7 (Plasmid #11795 at Addgene), pLB (Plasmid #11619 at Addgene), pWPXL (Plasmid #12257 at Addgene), pWPI (Plasmid #12254 at Addgene), EF.CMV.RFP (Plasmid #17619 at Addgene), pLenti CMV Puro DEST (Plasmid #17452 at Addgene), pLenti-puro (Plasmid #39481 at Addgene), pULTRA (Plasmid #24129 at Addgene), pLX301 (Plasmid #25895 at Addgene), pHIV-EGFP (Plasmid #21373 at Addgene), pLV-mCherry (Plasmid #36084 at Addgene), pLionII (Plasmid #1730 at Addgene), pInducer10-mir-RUP-PheS (Plasmid #44011 at Addgene).

    Clone Assay:

    Article Title: Failure to repair damaged NAD(P)H blocks de novo serine synthesis in human cells
    Article Snippet: .. Both the isoforms were cloned from pcDNA3.1 that contained the human full-length NAXD cDNA (RefSeq ID: NM_001242882.1; GenScript clone ID OHu08429) [ ]. pLX301 was a gift from David Root (Addgene plasmid # 25,895) [ ]. .. The lentiviral particles were generated in human embryonic kidney (HEK) 293 T cells via co-transfection of three plasmids (recombinant pLX301, containing the eGFP, CytoNAXD or MitoNAXD inserts, pCMV-δ8.2, and pCMV-VSVg) using LipofectamineTM 2000 transfection reagent (Invitrogen), as detailed previously [ , ].

    Article Title: An enhanced intracellular delivery platform based on a distant diphtheria toxin homolog that evades pre-existing antitoxin antibodies.
    Article Snippet: .. Similarly, to generate lentivirus for HEK293T SORT1 overexpression cell lines, SORT1 cDNA was obtained from the hORFeome V8.1 collection and cloned into pLX301 (Addgene #25895) for co-transfection with psPAX2 and pVSV-G (Addgene #138479). ..

    Over Expression:

    Article Title: An enhanced intracellular delivery platform based on a distant diphtheria toxin homolog that evades pre-existing antitoxin antibodies.
    Article Snippet: .. Similarly, to generate lentivirus for HEK293T SORT1 overexpression cell lines, SORT1 cDNA was obtained from the hORFeome V8.1 collection and cloned into pLX301 (Addgene #25895) for co-transfection with psPAX2 and pVSV-G (Addgene #138479). ..



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